FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_A9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_A9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6899121
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG119450.1731379983044217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC77250.024.7765057
ATACAGG117450.024.3207956
TATACAG149800.022.9888045
ACAGGCA85750.021.9823348
GAGCGCA23550.020.6261639
CAGGCAA94900.019.6336699
TAGAGCG26150.018.2981347
GTATATA240250.017.748251
GTATTAG62050.017.41351
TATATAC276650.016.273813
TGCAGTC32050.016.0607157
TATACTG68800.016.0173155
ATATACA253950.015.9584934
TACAGGT56600.015.7551767
ATTATAC129950.015.5094643
CTCTGTG46450.015.4519767
CTAATAC77400.014.9867453
TAGGTAG34650.014.8555815
GTATAGA76750.014.4562181
TATACCG41800.014.0489335
Produced by FastQC (version 0.11.5)