FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_A8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_A8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742438
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG36660.13367667746727546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCA9600.028.6976439
TACAGGC22250.028.3480457
ATACAGG39200.025.892616
TAGAGCG11950.025.4809287
TAGGTAG17500.024.8569055
TATACAG58100.024.0832445
CAGGCAA27900.023.6467279
ACAGGCA29000.022.4997838
GTATTAG26600.022.0824931
GTAGTCA18550.020.7140878
TCGTACG5403.6379788E-1220.1386977
TGCAGTC10450.019.4256537
TAGTCAG20550.019.4037089
GTATATA103250.019.1039681
CAGCGTG8400.018.9879159
GTATAAT48950.018.962781
CGTACGT7050.018.5104628
TACAGCG11600.017.4998327
GTATACA41750.017.3695371
TACAGGT20450.016.6624327
Produced by FastQC (version 0.11.5)