FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_A7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_A7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6165830
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG148940.24155709774677536No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA88790.14400332153173212No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT68610.11127455671012662No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT68090.11043119904376217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGG127050.028.4734086
TACAGGC78900.028.3000567
TGCAGTC29600.027.6758087
TATACAG158100.026.9165765
ACAGGCA88800.025.4715398
CAGGCAA95000.023.7328769
TAGAGCG23750.023.503947
GAGCGCA23450.023.1863279
TACAGGT62550.021.7893417
GTATATA235350.020.7692741
CTCTGTG45200.020.369437
GTATTAG57600.019.0120981
TATACCG41050.018.8966245
ATTATAC129900.018.361173
TAGGTAG33650.018.312495
TATATAC282500.018.194553
TCGTACG9800.017.7541037
ATATACA242550.017.6642934
GTATAAT103500.017.3774851
ACCGATG33850.017.1334538
Produced by FastQC (version 0.11.5)