FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_A6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_A6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1982907
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG22200.11195683912558682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG3950.027.5307887
GAGCGCA7050.024.6800829
ATACAGG28150.022.1485126
TGCAGTC8250.021.9690157
TACAGGC17600.021.0078687
TATACAG37600.020.823825
ACAGGCA19000.020.2230558
GCGTGTC1800.00430931620.1382629
CGTACGT5453.6379788E-1219.9535068
TAGAGCG9900.019.0398127
GTATAAT37350.018.0584771
TAGACTC8250.017.575215
CAGGCAA22050.017.0969739
CCGCTTA6607.4578566E-1116.476343145
TTAGACT12000.016.3111694
TACAGCG8550.016.110617
TATATAC78000.015.9866813
CTCTGTG11450.015.8292027
TATACCG11550.015.6921535
GTATATA71650.015.6893261
Produced by FastQC (version 0.11.5)