FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_A5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_A5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290900
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG63830.19395909933452854No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA41470.12601416026011122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCA13250.028.9993889
TACAGGC39150.026.295747
ACAGGCA42350.024.9935688
TAGAGCG16000.024.9213477
ATACAGG60300.024.7673026
TATACAG79500.022.4334875
CAGGCAA48100.022.0057729
GTATAAT62750.021.1494061
TCGTACG7000.020.7138487
CAGTGTC19200.019.6350029
CAGCGTG8350.019.101399
GTATATA123300.019.0564821
CTGTCAG10050.018.7558235
TGCAGTC15050.017.8235447
CCTAGGA3352.8582323E-517.3156972
ACAGCGT11400.017.1706898
CGTACGT9900.016.8430798
GTATACA41650.016.715411
TACAGGT27900.016.6304727
GGAACTT62550.016.574139145
Produced by FastQC (version 0.11.5)