FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_A4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_A4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7637998
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG267490.35020957062308733No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA147170.1926813806445092No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT116900.15305057686582269No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT106000.13877982162341493No Hit
GATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTATTC94940.12429958740497182No Hit
ATATATTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCC87250.11423150411927314No Hit
GTTATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGAG83770.10967533639050442No Hit
ATATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCT79660.10429434519359654No Hit
CTATAATGCAGTCTCTTGATAACTTTTTGCACTGTAGGTCCGTTAAGGTT78210.10239594197327626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAGTC40800.035.7156567
TACAGGC124750.032.3696027
ATACAGG192350.031.0568736
ACAGGCA130700.030.5631928
TATACAG219050.029.7535745
GAGCGCA25000.028.129019
GCCGACC4400.028.010197145
CAGGCAA148950.027.1104939
TAGAGCG30400.023.1324087
CACCCGC28250.023.09638145
TACAGGT81650.022.4639977
ATGCAGT78700.021.0030736
GTATATA325000.020.3295781
TATATAC396700.019.5729223
ATATACA323400.019.4579354
ACCGATG46550.019.0004068
TATACCG50050.018.9753555
CTCTGTG67400.018.715967
ATTATAC164100.018.6437073
ACAGGTT99700.018.6151928
Produced by FastQC (version 0.11.5)