FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_A3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_A3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4915237
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG83510.16990025099501815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC49250.027.6743627
ATACAGG80650.025.7091466
GAGCGCA17250.025.6369729
ACAGGCA56800.023.8681778
TATACAG104900.023.2214765
TAGAGCG19650.022.8746877
CAGGCAA60850.022.0412989
TGCAGTC20200.021.5340587
GTATATA176300.020.2375581
GTATTAG49000.018.6474651
TAGGTAG28900.018.3126435
TATATAC201300.018.295573
CAGTGTC22550.017.3609399
CACCCGC15700.017.085167145
GACTAGG14900.017.0297328
GTATAAT88950.016.6314281
ATATACA171250.016.5946564
TATACCG27100.016.3187375
GTAGTCA26250.016.2947888
TCTATAC52550.015.4515233
Produced by FastQC (version 0.11.5)