FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_A2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_A2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4857895
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG144760.2979891496213895No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA85330.17565221150313048No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT69690.14345719699581813No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT61710.12703032897993885No Hit
ATATATTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCC55970.11521451163518355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGG120150.028.3595336
GAGCGCA19950.028.3450169
TGCAGTC24450.027.8723777
TACAGGC76950.027.6989777
TATACAG145450.026.7162865
ACAGGCA82450.025.9391888
TAGAGCG21850.025.8802347
TACAGGT51100.024.5442737
CAGGCAA90750.023.3271269
CTCTGTG35950.021.1746087
GTATATA197600.020.4784681
GTATAAT83100.020.2459031
ACAGGTT66650.020.2319538
CTGTCAG13350.020.0930335
TCGTACG9100.019.9169867
GTATTAG41750.019.4541261
TCGAATA58350.019.382256145
GGAACTT111400.019.002827145
ATTATAC107050.018.9625343
CAGCGTG11200.018.7717599
Produced by FastQC (version 0.11.5)