FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_A1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_A1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4333548
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG110210.25431817069985146No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA65560.15128481327540388No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT57430.13252420418557725No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT46980.10841001415006826No Hit
ATATATTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCC45440.1048563440395722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC59950.028.6602737
ATACAGG95850.028.060996
ACAGGCA65650.025.7301548
TGCAGTC20300.025.713297
TATACAG117350.024.6496625
CAGGCAA71150.023.8430679
GAGCGCA15650.022.6988019
TACAGGT44450.021.6920747
GTATTAG38450.021.1223931
TCGTACG9650.020.284197
GTATATA176150.019.7596841
TAGAGCG17350.018.8033077
CTGTCAG12850.018.6179545
GTATACA54350.018.0117381
GTATAAT80000.017.9471971
ATTATAC93250.017.8035983
TAGGTAG23250.017.7735045
CTCTGTG33150.017.4955527
GGAACTT94650.017.463665145
CGTACGT11650.017.424228
Produced by FastQC (version 0.11.5)