FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n02_msh3_MA_strain_A11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n02_msh3_MA_strain_A11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4481714
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG90090.20101684310957815No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA55340.12347954376383677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGG88950.026.1621196
TACAGGC55300.025.694777
TATACAG110800.024.6015175
GAGCGCA18600.023.7755579
ACAGGCA61400.022.6697468
TGCAGTC21200.022.5694967
TAGAGCG21100.020.9585487
CAGGCAA66050.020.7444929
GTATATA171450.020.2171761
CTCTGTG30650.019.8683877
TAGGTAG23100.019.1439555
CAGCGTG11750.019.1265939
TACAGGT40650.019.0825777
GTATTAG39700.018.2658391
TATATAC194600.017.546363
GTATAAT79350.017.363481
ATTATAC88900.017.1248383
ATATACA170300.016.3037784
TCGAATA44500.016.291046145
ATCTCTG40100.015.9093375
Produced by FastQC (version 0.11.5)