FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11350155
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1914511.6867699163579706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC49450.033.0886737
ATACAGG76850.030.3100786
TATACAG98300.028.3913355
TAGGTAG23500.024.243075
GTATATA153700.022.4857541
TATATAC183300.021.2451443
ATATACA173450.020.3727724
TGCAGTC20600.020.2946017
GTATTAG39350.020.211631
GTAGTCA24900.019.9836258
GGAACTT75300.017.71836145
TTCAGCC29550.017.422142145
GACTAGG13300.016.8087188
CCGATCG6104.2564352E-1016.641747145
CTAATAC48350.016.5559793
TCTATAC41850.016.0256583
CCGACGC8601.8189894E-1215.17661145
TATATAG126950.015.1104465
TACTATG54600.014.6537957
ATACTAT99950.014.5746116
Produced by FastQC (version 0.11.5)