FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_s.EP062.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_s.EP062.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3890260
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG79790.20510197261879667No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA39930.10264095458915343No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC43650.032.387757
ATACAGG67900.031.2932786
TATACAG89100.028.6495725
CAGGCAA52700.026.5507669
GAGCGCA14750.026.5419069
TAGAGCG15000.025.132897
TAGGTAG19750.024.9686455
CTGTGCG5850.022.30739
TACAGGT31700.021.9554527
GTATTAG39000.021.20121
TGCAGTC17050.020.8354117
GACTAGG14200.020.4221788
TATATAC167150.019.0029473
GTATATA141550.018.9076141
ATATACA144850.018.8745254
GTAGTCA25750.018.58228
GGAACTT72700.017.750721145
CTAATAC52100.017.6774333
TACTATG54350.017.4743947
ATTATAC79150.017.1334113
Produced by FastQC (version 0.11.5)