FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_s.EP060.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_s.EP060.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4607292
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG96820.21014513514663277No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA48150.1045082447563558No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC54200.030.764947
ATACAGG83050.028.6404346
GAGCGCA15550.027.5073769
ACAGGCA59500.027.4153068
TATACAG103400.027.2117565
CAGGCAA64400.025.2167849
TGCAGTC22000.024.0562387
TAGAGCG18650.022.9351027
TACAGGT38900.018.6370837
TATATAC196000.017.9814873
GTATATA164100.017.856581
CACGAGC13000.017.845722145
ATTATAC91150.017.6620733
GGAACTT86850.017.5298145
ATATACA169300.017.2620944
GTATAAT81250.017.1397061
GACTAGG10850.016.7046668
ATGCAGT40350.015.8155586
GTATTAG39650.015.3660281
CAGCGTG9500.015.262799
Produced by FastQC (version 0.11.5)