FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_s.EP050.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_s.EP050.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13168842
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG482900.3666989094409364No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA259210.196835834160665No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT193730.14711240365705658No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT175760.13346655689239798No Hit
ACCTAATACTATGCAAAAATGTAAAATCATCACACAAAACATAAACAATC161750.12282780824616164No Hit
GATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTATTC158730.12053451624675883No Hit
GTATTAGGTAGTCAGATGAAAGATGAATAGACATAGGAGTAAGAAAACAT156670.11897021773060988No Hit
ATATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCT134890.10243117807928745No Hit
CTATAATGCAGTCTCTTGATAACTTTTTGCACTGTAGGTCCGTTAAGGTT133630.10147437413251673No Hit
ATATATTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCC131690.10000119980177452No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC201650.035.4850777
ATACAGG314950.032.9992876
TAGGTAG77950.032.5650635
TGCAGTC64400.031.9712247
ACAGGCA226600.031.6099538
TATACAG386850.029.6220075
CAGGCAA245550.029.17059
GTAGTCA88150.028.0452238
GAGCGCA40650.027.287129
TACAGGT111850.026.2510017
TAGAGCG45450.025.3623837
GTATTAG138750.025.1471981
TCTCTGA92800.023.905634145
CAGCGTG39800.023.4981389
GGATACG63200.022.7130229
TACTCGG24950.021.502457
ATATACA576800.021.1243824
ACAGCGT46500.021.0478468
GTATATA532200.020.990531
TATACCG78250.020.947115
Produced by FastQC (version 0.11.5)