FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_s.EP049.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_s.EP049.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7491862
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG128700.1717863996960969No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC77100.026.4229567
ATACAGG116450.026.0957156
TATACAG153800.023.76675
ACAGGCA86900.023.1928658
CAGGCAA93150.021.4810569
GTATTAG69250.019.7970521
TGCAGTC35150.017.119157
GTATATA262400.016.8902421
TATATAC314650.016.6880473
ATATACA259200.016.4526944
TACAGGT59100.016.3152457
CAGCGTG18100.016.0217849
GAGCGCA18900.015.7272039
TCGTACG13400.015.6899897
CACCCGC21700.015.36836145
ATTATAC150900.015.2838433
TAGGTAG46050.015.2769545
TATACCG41700.014.9574345
GACTAGG17950.014.94399458
GGAACTT129600.014.768227145
Produced by FastQC (version 0.11.5)