FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_s.EP026.2A-2C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_s.EP026.2A-2C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8760606
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG78500.014.6927361
GTATATA246350.014.5462011
GTATAGA82700.014.121981
CTAATAC85050.014.0707693
TATACGG80600.013.6778155
TATACTG119800.013.5612415
TATATAG155800.013.5001555
TTATACT152500.012.60334
GTATAAT160050.012.5998181
CCTATAC46850.012.5395993
ACTATGC67300.012.4959058
CTATACT90800.012.4608314
TATATAC277050.012.3825823
TATACAG105800.012.2708765
TCTATAC80500.012.0730543
ATTATAC164000.012.0291033
ATACGGT79200.011.9964946
CAGCGTG22450.011.9484119
CATATAC135450.011.9408193
TAGACAG47750.011.8475755
Produced by FastQC (version 0.11.5)