FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_D2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_D2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2573692
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG53640.20841654712374286No Hit
ACCTAATACTATGCAAAAATGTAAAATCATCACACAAAACATAAACAATC30310.11776855971887855No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA28720.11159066430637388No Hit
GATATATACAATACATGTTTTTACCCGGATCATAGAATTCTTAAGACAAA26150.10160500945723108No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCG7850.028.6295957
TACAGGC29000.028.4990257
GAGCGCA7450.028.2205149
CAGCGTG8250.028.1202539
TAGGTAG16550.026.7310075
GGATACG12500.026.6790899
ATACAGG45550.025.9527116
TATACAG63350.024.7280965
ACAGCGT10700.023.7141428
GACTAGG10900.023.2790228
CAGGCAA34750.023.1574829
GTAGTCA18500.023.120838
ACAGGCA36050.022.9257078
GTATTAG27450.022.7251721
TACAGCG12800.020.9563167
TAGTCAG21450.020.9549949
GTATAAT38400.020.022841
CTTACAC13200.019.7793543
TACTATG37950.019.1034327
CTAATAC39700.018.8161283
Produced by FastQC (version 0.11.5)