FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_D1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_D1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5749198
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG110930.19294865127275143No Hit
ACCTAATACTATGCAAAAATGTAAAATCATCACACAAAACATAAACAATC70740.12304324881487819No Hit
GTATATATCTATTATAATATACGATGAGGATGATAGTGTGTAAGAGTGTA61200.10644963001796076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC60750.030.073067
CAGCGTG19900.029.5090569
TAGGTAG33500.027.7133315
TATACAG130700.026.748235
ATACAGG99350.026.0629716
ACAGCGT23100.026.0489048
ACAGGCA72950.025.3418458
GGATACG31400.024.2428139
TACAGCG29200.024.0830947
CAGGCAA75100.024.037149
GAGCGCA16900.023.1649029
GTATTAG61950.021.7795121
GACTCCG9700.021.674528145
GACTAGG27750.021.42278
TGCAGTC24750.020.5043587
TAGAGCG18750.020.4926417
GTAGTCA42450.020.3232258
TATATAC234100.020.2318383
GTATATA202450.020.1370121
ATATACA213600.019.0835274
Produced by FastQC (version 0.11.5)