FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_C2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_C2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6973891
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG173940.24941600033611078No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA91930.13182024210014182No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT70500.10109134197824428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC90350.033.3806957
ATACAGG141750.031.1071516
ACAGGCA100300.029.0573048
TATACAG175550.027.7618335
CAGGCAA108150.027.0152329
GAGCGCA22550.024.112659
TACAGGT61150.021.9333767
CAGCGTG15750.021.1742259
TAGAGCG25550.020.9976657
TGCAGTC33000.020.6511447
ATATACA271100.019.5554374
TATATAC324500.019.5109883
GTATTAG62250.019.110061
GGAACTT147800.019.032135145
TAGGTAG37000.019.012965
GTATATA265950.018.3830471
CTAATAC80050.017.7571983
ATTATAC150100.017.249133
GACTAGG18150.017.1759998
GTATAAT121050.016.8383331
Produced by FastQC (version 0.11.5)