FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_C1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_C1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3930322
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG105590.2686548328610226No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA63210.16082651752197402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC56100.030.1108867
GAGCGCA16850.028.8273939
ATACAGG86200.027.8466366
TAGAGCG19150.027.2580137
TATACAG106200.026.0167165
CAGGCAA67050.025.0853239
GACTAGG10550.024.0517148
TGCAGTC19950.021.8041327
GTATTAG33750.020.4153651
GTATATA154150.020.0434911
GGAACTT83350.019.918669145
GTATAAT70100.019.7616251
TATATAC181750.019.391613
ATATACA160500.018.7962114
TACAGGT36150.018.2500297
CTCTGTG28900.018.061977
CAGCGTG10300.017.596789
CTAATAC42600.017.3637123
CTACCCC16000.017.2232724
ATAGAGC33050.017.1149316
Produced by FastQC (version 0.11.5)