FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_B2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_B2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5115281
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG280830.5490020978319665No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA165050.3226606710364494No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT122990.24043644914130816No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT106130.20747638301786353No Hit
ATATATTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCC86800.1696876476580661No Hit
ATATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCT77500.15150682826613043No Hit
GATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTATTC77090.15070530827143222No Hit
CTATAATGCAGTCTCTTGATAACTTTTTGCACTGTAGGTCCGTTAAGGTT75560.14771427024243633No Hit
TATATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGC63860.12484162649129148No Hit
GTATATATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTG63160.12347317771985546No Hit
GTTATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGAG62910.12298444601577117No Hit
GCATTATAGAGCGCACAAAGGAGAAAAAAAGTAATCTAAGATGCTTTGTT61060.11936783140554742No Hit
GTATAATAATATATAGTCTAGCGCTTTACGGAAGACAATGTATGTATTTC60380.11803848117043815No Hit
ACATAAATAGACGCATATAAGTACGCATTTAAGCATAAACACGCACTATG59010.11536023143205623No Hit
ATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGAGCAA57670.11274062949816442No Hit
ATATAGAACAGCTAAAGGGTAGTGCTGAAGGAAGCATACGATACCCCGCA56070.10961274659202495No Hit
GATATAGGTGCGACGTGAACAGTGAGCTGTATGTGCGCAGCTCGCGTTGC55980.1094368031785546No Hit
ATACTAGTATACTCCGTCTACTGTACGATACACTTCCGCTCAGGTCCTTG55380.10826384708875231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCA27350.036.050249
TAGAGCG28350.035.0343557
ATACAGG190850.034.0129476
TACAGGC125000.033.871117
TATACAG211350.032.841515
TACAGGT67750.032.4235047
ACAGGCA131500.031.9212138
TGCAGTC36350.031.7116857
CAGGCAA145500.028.7002759
TCGAATA77150.027.815212145
TAGGTAG20950.027.0037445
ACAGGTT85600.026.5092338
ATTATAC106750.026.3619333
GGAACTT159950.025.654219145
CTCTGTG49300.024.9993427
ATAGAGC42850.024.7124316
GTATAAT72050.024.1627241
GTATATA239700.023.3624231
CACCCGC24100.023.16329145
TATACCG37600.022.9547485
Produced by FastQC (version 0.11.5)