FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_B1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_B1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3748104
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG100050.26693496231694747No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA58720.15666587693404452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCG14300.029.911787
GAGCGCA14350.029.3023439
TACAGGC50950.028.0316267
GACTAGG13550.026.7520438
TGCAGTC17700.026.213987
ATACAGG77800.026.1015876
TATACAG99950.025.6141785
TAGGTAG18500.024.3057175
ACAGGCA59500.024.1253978
CAGGCAA61300.022.9439149
TATACCG20850.020.870545
GTATTAG36550.020.8370781
CAGCGTG12050.019.8542359
TAGGACT14050.019.6153374
TATACTG35600.019.5573395
CTAATAC50650.019.4736773
GTATATA143250.019.3927351
ATAGTCT41100.019.0505818
TATATAC170400.018.9825173
GTATAGA48700.018.4683281
Produced by FastQC (version 0.11.5)