FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_A9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_A9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6899121
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG140760.20402599113713182No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA73240.10615845119979776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC78550.025.750567
ATACAGG120300.025.2609836
TATACAG154000.024.1600845
ACAGGCA87000.022.4995178
GAGCGCA25000.020.5895589
CAGGCAA99100.019.6791939
TAGAGCG26250.019.6091027
GTATATA239900.019.2602271
GTATTAG64000.017.9072671
TAGGTAG33900.017.7574355
CAGCGTG19500.017.4739849
TGCAGTC32400.017.2295687
TATATAC282750.016.8268593
TACAGGT58250.016.8022147
TCTATAC68700.016.4690933
ATTATAC122650.016.0252043
GACTAGG19150.015.9004438
ATATACA254950.015.418644
GTATAAT108200.014.7484921
TAGGACT26600.014.4509394
Produced by FastQC (version 0.11.5)