FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_A8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_A8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742438
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG46250.1686455628167346No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA31570.11511654958106618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG16600.027.5287045
TACAGGC25750.027.028237
ATACAGG42750.024.0938136
TATACAG61150.023.1308445
CAGGCAA31400.022.3957569
GAGCGCA8100.022.3758039
GTATTAG25000.022.0537471
GACTAGG9950.021.8585748
TGCAGTC10650.021.7833167
GTAGTCA17700.021.708328
TACTATG37450.021.100777
CTATGCA37950.020.0586269
GTATAAT48500.019.445111
CTAATAC35650.019.3293573
TAGAGCG9500.019.0783187
TAGTCAG21350.018.6761979
TCGTACG6301.8189894E-1218.412097
ATATACA98600.018.0236384
AGACTAG12250.017.7545177
CCGTGCG2050.00913208617.682349
Produced by FastQC (version 0.11.5)