FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_A7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_A7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6165830
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG176700.28657942239730905No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA97800.15861611494316255No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT67880.11009061229388421No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT66890.10848498904445954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC88900.031.233827
ATACAGG133950.028.7481736
ACAGGCA97750.028.4068
TATACAG162450.026.561695
GAGCGCA20800.026.1412289
CAGGCAA109600.025.2685839
TGCAGTC31000.024.5558937
TAGAGCG23350.023.5968917
GTATATA238500.019.887951
TACAGGT56700.019.8187717
GGAACTT134150.019.779646145
TATATAC289300.018.9259173
ATATACA258050.018.91344
GACTAGG17000.017.9113568
GTATAAT104300.017.7319531
TAGGTAG28750.017.6570685
TATACCG39850.017.652325
ATTATAC121200.016.514483
GTATTAG55750.016.1316031
CTACCCC24900.015.7272524
Produced by FastQC (version 0.11.5)