FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_A6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_A6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1982907
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG28380.14312320244973667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC18950.024.1021087
GTATTAG17800.023.6323551
ATACAGG29100.023.1775256
ACAGGCA22550.020.8973188
TCGTACG4051.8853825E-819.690717
TATACAG40650.019.446575
CAGGCAA23150.019.416219
TAGGTAG11600.018.1308045
GTATAAT37950.017.9644991
GACTAGG5701.6189006E-1017.806438
GTATATA71550.016.9280051
CTAATAC21750.016.6720053
TACGGCG2650.002059175416.4145557
TATACGG18200.016.3376485
TATATAC81400.016.304363
TACGGTG13400.016.230817
ACGGTGT14300.016.2232448
TAGAGCG6459.422365E-1015.7359177
ATATACA69050.015.6494714
CTGTCAG7654.1836756E-1115.1682555
Produced by FastQC (version 0.11.5)