FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_A5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_A5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3290900
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG81300.2470448813394512No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA45400.13795618219939834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCA12950.030.7909139
TACAGGC42450.030.570677
ACAGGCA46800.027.109528
ATACAGG64400.026.464086
TAGAGCG15500.025.725317
CAGGCAA51650.024.5639029
TATACAG81400.024.500965
GTATTAG27500.020.3113371
TATATAC142150.019.5400983
GTATATA119150.019.3603651
GTATAAT61350.019.2731841
ATATACA123200.019.2491654
GTATACA42550.019.094091
CTCCCCG4506.2664185E-817.732121
TATACCG19450.017.52485
GGAACTT70850.017.497898145
GACTAGG8800.017.3008028
TAGGTAG18800.016.9734335
ATAGAGC27450.016.9087876
CAGCGTG7950.016.4147789
Produced by FastQC (version 0.11.5)