FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_A3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_A3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4915237
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG101570.2066431384692132No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA53460.10876382969936138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC55500.028.4769277
ATACAGG89050.025.5710776
TATACAG115700.024.0059415
GACTAGG15400.023.5385078
CAGGCAA68850.023.2711549
GAGCGCA17950.021.8101639
TAGAGCG19250.020.3372697
TATATAC216000.019.640633
GTATATA180750.019.261361
GTATTAG47450.019.2600921
ATATACA179600.018.2913254
TGCAGTC21600.018.1246477
GTATAAT91550.017.9842191
CAGCGTG13600.017.5915729
GGAACTT90600.017.044373145
TAGGTAG29900.016.7352125
TACAGCG20950.016.26467
GTATAGA61000.016.170861
TACAGGT41150.015.8563157
GCCTTAG22100.015.7533531
Produced by FastQC (version 0.11.5)