FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_A2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_A2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4857895
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG177640.36567278625824556No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA94740.19502274133137912No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT69430.14292198575720555No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT63110.12991223564939136No Hit
ATATATTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCC49840.10259587743250934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC85400.030.3915467
GAGCGCA19100.030.3657659
ATACAGG127150.028.6909316
ACAGGCA91700.028.2245168
TAGAGCG21350.026.8260577
CAGGCAA98300.026.4032349
TATACAG155900.025.8190295
CTGTCAG13500.023.6380655
TGCAGTC26400.023.616867
TACAGGT48900.021.7939577
TCGTACG8650.021.7913867
GGAACTT115350.021.746338145
GTATTAG44000.021.2665561
GTATATA205200.020.2905881
TATATAC249700.019.3731543
TAGGTAG25950.019.2843385
CACCCGC17300.018.85793145
GTATAAT85000.018.7743051
ATATACA216700.018.7088014
GTATACA61950.018.6172681
Produced by FastQC (version 0.11.5)