FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_A1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_A1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4333548
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG138650.3199456888443373No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA74070.17092230200288538No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT56640.13070121757045267No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT47690.11004839452568659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC69100.033.4688577
ATACAGG101650.032.602986
ACAGGCA74900.030.8771428
GAGCGCA14050.030.4441579
TATACAG128800.029.0524225
CAGGCAA82850.027.9142789
TAGAGCG15850.027.4441767
TGCAGTC20800.026.8383377
GGAACTT91400.024.58916145
TACAGGT38900.022.9236497
ATATACA179600.021.3194544
TCGTACG9150.020.6006287
TATATAC216950.020.2229373
GTATAAT81400.018.6215381
GTATATA176250.018.5995711
TAGGTAG22550.018.3306065
GTATTAG38100.018.2742631
TAGGACT13250.018.06124
CTAATAC47600.017.9772623
ATTATAC96500.017.8853783
Produced by FastQC (version 0.11.5)