FastQCFastQC Report
Wed 7 Oct 2020
HH2JLAFX2_n01_msh3_MA_strain_A11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2JLAFX2_n01_msh3_MA_strain_A11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4481714
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG107740.24039909730964537No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA61640.13753666565961148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGC58950.028.7775617
GAGCGCA16950.026.5181949
ACAGGCA64300.026.3831628
TAGAGCG18350.026.075337
TATACAG111050.025.9305675
ATACAGG88600.025.869826
TGCAGTC20350.025.2939057
CAGGCAA69000.024.2708429
TATATAC207350.020.1842143
ATATACA176950.019.4297774
GGAACTT89550.018.701153145
CAGCGTG11650.018.6688399
GTATAAT76200.018.566551
GTATATA174450.018.4656071
GTATTAG39950.017.6159691
GACTAGG13500.017.1845518
CTAATAC53100.016.9381983
ATAGAGC34350.016.8928646
TAGGTAG25800.016.868315
GTGCTCC16700.016.4963979
Produced by FastQC (version 0.11.5)