FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10965851
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT393150.060.3644512
GCACACG395400.060.0654611
ACGTCTG379500.059.91304815
CGTCTGA380250.059.67709716
CACGTCT381050.059.37285214
ACACGTC386250.059.3209313
AGCACAC416900.058.1968110
CTGAACT392400.057.81442619
AGAGCAC434850.056.9843148
GAACTCC410250.055.61155321
GATCGGA455100.055.3916631
CGGAAGA460400.055.3348434
TCGGAAG458850.055.2884453
TGAACTC421350.054.9757820
CTCCAGT384950.054.44920724
ATCGGAA468800.054.420652
AACTCCA429700.053.96429422
CCAGTCA329900.053.34816426
TCTGAAC429250.053.33661718
GTCTGAA434000.053.05352817
Produced by FastQC (version 0.11.5)