FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_JK14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_JK14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19213797
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT15909458.280221759395085TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1719000.067.1267146
ATGCCGT1719550.067.0163849
TATGCCG1721950.066.9431248
GTATGCC1724900.066.8406447
CTCGTAT1725000.066.8223944
TGCCGTC1724800.066.7998750
ACGAGTG1731600.066.7669232
GCCGTCT1727100.066.6992751
CGAGTGG1729050.066.68136633
TCGTATG1731350.066.67837545
AGTCACG1752300.066.31174528
CACGAGT1749100.066.2491531
GTGGATA1738100.066.0655536
GTCACGA1759400.065.9828329
GGATATC1740250.065.9559438
TATCTCG1745250.065.9513141
CCGTCTT1750400.065.8397652
CTGCTTG1751200.065.7794259
GAGTGGA1751250.065.668234
TCACGAG1768300.065.5931630
Produced by FastQC (version 0.11.5)