FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_JK13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_JK13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18655141
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT5102952.735412184769871TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT563350.065.9463434
CCGTACG568450.065.564233
CGTATGC573400.065.4444746
TATGCCG576450.065.06264548
ATGCCGT577550.064.9203749
GTACGTA574000.064.9056935
GTATGCC580400.064.6251947
GCCGTCT580300.064.5486351
TGCCGTC582700.064.3179450
TACGTAA579850.064.2627736
CTCGTAT584050.064.088844
TCGTATG586950.063.94540845
CACCGTA591450.063.1746831
ACGTAAT591100.062.98658437
CCGTCTT600100.062.51135652
GTCACCG598450.062.50607729
ACCGTAC598000.062.47084432
AGTCACC604350.062.06330528
CGTAATC598800.062.0423738
GTAATCT599650.061.94842539
Produced by FastQC (version 0.11.5)