FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_JK12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_JK12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13755672
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT10575107.6878105264504715TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGTAT193660.1407855610398387TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGAT166750.12122272179796087TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1115200.067.665546
GTATGCC1123900.067.3968147
TATGCCG1109550.067.2937948
CTCGTAT1137450.067.25313644
AGTCACG1244900.067.2135328
ATGCCGT1103650.067.1873749
TCGTATG1119850.067.1631245
GTCACGT1243500.067.05939529
TGCCGTC1102250.067.056850
GCCGTCT1089350.067.0291451
CGTTTCG1242550.066.63673433
CGGAATC1233900.066.55669438
CCGTCTT1116500.066.51272652
CAGTCAC1260050.066.46651527
GTTTCGG1247300.066.35161634
TCTCGTA1161250.066.293743
GGAATCT1203800.066.28483639
CCAGTCA1263550.066.20689426
ACGTCTG1271500.066.1902615
TCCAGTC1265750.066.0752325
Produced by FastQC (version 0.11.5)