FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_JK11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_JK11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15240550
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT12215138.01488791415008TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTTA1277300.068.5047436
CGTATGC1299150.068.095946
GTATGCC1305000.067.7986947
ATGCCGT1304700.067.7693449
TATGCCG1304850.067.7481448
CGTGGCC1298250.067.6895433
TGCCGTC1306600.067.66119450
GTCACGT1311450.067.6140229
AGTCACG1313500.067.6124128
TCGTATG1308400.067.5933145
CTCGTAT1307900.067.5578244
GCCGTCT1307650.067.5270151
GCCTTAT1296400.067.47632637
ACGTCTG1324650.067.4391715
ACACGTC1330200.067.2571113
CGTCTGA1328900.067.2208516
GCACACG1332700.067.1863211
CACGTCT1330600.067.1801314
CACACGT1333800.067.1256512
GTGGCCT1304800.067.1186234
Produced by FastQC (version 0.11.5)