FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_AKM143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_AKM143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20130019
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT10177825.056040930711491TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1182000.066.8795246
TCGTATG1183050.066.76707545
CTCGTAT1178600.066.7311244
GTATGCC1189650.066.4198747
TATGCCG1196950.066.0097648
GCCGTCT1200200.065.7522751
TCTCGTA1195200.065.6403243
ACGATCT1166850.065.26733439
AGTCACG1213950.065.2475928
CCGTCTT1212800.065.1360252
AACGATC1170550.065.063738
CGATCTC1173900.065.02492540
GTCACGT1217250.064.9678429
CACGTGA1215350.064.9193431
ATGCCGT1219050.064.79584549
GATCTCG1204550.064.7588941
ATCTCGT1210800.064.6844442
TCACGTG1223850.064.55424530
TGCCGTC1228100.064.3373550
CAGTCAC1227300.064.32683627
Produced by FastQC (version 0.11.5)