FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_AKM142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_AKM142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24225733
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT3819401.5765880025178185TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC474000.062.37024746
GTATGCC478350.061.84696247
TATGCCG479150.061.72922548
CTCGTAT479350.061.55744644
GTCCGCA468350.061.45559334
CGTCCGC471000.061.3254433
TCGTATG483200.061.1827345
ATGCCGT485350.060.8977949
GCCGTCT487400.060.64933451
TCCGCAC472000.060.60973435
CGCACAT474000.060.487437
ACGTCCG481900.060.48278432
TGCCGTC493300.059.9167450
CCGCACA479500.059.6403136
CACGTCC499400.058.468731
CCGTCTT507350.058.3063652
TCTCGTA513000.057.54007743
GCACATC504700.057.25916338
CTGCTTG516400.057.15740659
CATCTCG514700.056.5818841
Produced by FastQC (version 0.11.5)