FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_AKM141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_AKM141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20865377
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT7851693.763023308900673TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG866550.066.4600335
CGTATGC888150.066.2497346
GTCCCGA872250.066.1831736
CCCGATC869250.065.94388638
TATGCCG893900.065.7701948
CCGTCCC877400.065.7498834
GTATGCC894300.065.74061647
ATGCCGT896000.065.6274449
TGCCGTC898200.065.4561150
TCGTATG899650.065.4030445
GCCGTCT899550.065.3390551
CTCGTAT895650.065.2573244
CCCGTCC889250.065.1334733
ACCCGTC900650.064.54172532
CCGATCT889400.064.48907539
GATCTCG897800.064.4472241
CGATCTC894650.064.4040440
TCCCGAT897900.064.21052637
CACCCGT905500.064.20763431
CCGTCTT923450.063.686252
Produced by FastQC (version 0.11.5)