FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_AKM140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_AKM140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17904533
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT207164511.570505636756904TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTAT193780.10822957515842498TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2275950.067.5668646
TCGTATG2273000.067.4990345
GTATGCC2283950.067.4922547
CTCGTAT2298650.067.41881644
TATGCCG2268600.067.3653548
ATGCCGT2269000.067.0062249
TCTCGTA2329950.066.8618143
GAATCTC2367100.066.7498740
CCGTCTT2279250.066.6881452
AATCTCG2361550.066.6592541
GCCGTCT2257750.066.59247651
TGCCGTC2277050.066.5791250
CTGCTTG2233450.066.51633559
ATCTCGT2357650.066.4308642
CAGTCAC2449550.066.40082627
ACTCCAG2443450.066.3732823
AGAATCT2392450.066.3682739
ATGTCAG2430500.066.3638834
ACATGTC2438600.066.3628332
TGTCAGA2428100.066.3590235
Produced by FastQC (version 0.11.5)