FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_AKM139.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_AKM139.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19291715
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT308879416.010987099902728TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT3315550.067.94166649
CTCGTAT3315300.067.8642844
CGTATCT3293800.067.8615139
GTTCCGT3292750.067.860335
CGTATGC3323700.067.8428146
GTATGCC3327300.067.8151847
TCCGTAT3304300.067.761537
CCGTATC3298850.067.7609938
TTCCGTA3307850.067.72739436
TATGCCG3327400.067.7219748
TGCCGTC3325100.067.69250550
AGTTCCG3306850.067.6446934
TCGTATG3333900.067.6065645
TCCAGTC3360350.067.60071625
CAGTTCC3327050.067.59357533
GAACTCC3381300.067.5708921
ACTCCAG3366800.067.55334523
CTGCTTG3320500.067.5343659
CCGTCTT3333650.067.470652
GCCGTCT3330950.067.4624151