FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_AKM138.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_AKM138.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17870653
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT193802810.844752007663066TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2094850.067.6170246
GTATGCC2098900.067.4667247
TCGTATG2101550.067.4016345
CTCGTAT2093850.067.3969144
GTCACAG2108700.067.3281729
CACAGTC2106300.067.3234631
ACACGTC2141650.067.2978313
TATGCCG2104050.067.28680448
GAACTCC2129000.067.2692121
CACGTCT2139150.067.2559814
ACGTCTG2137700.067.2093415
ATGCCGT2107900.067.1628149
GCCGTCT2106800.067.1561651
CGTCTGA2140300.067.1197116
ACTCCAG2126050.067.0869423
GCACACG2148800.067.0767811
AGTCAAC2102450.067.0664634
CACACGT2150400.067.0301312
TCCAGTC2125000.066.990325
TGCCGTC2113450.066.9814750
Produced by FastQC (version 0.11.5)