FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_AKM137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_AKM137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16667868
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC16045259.626456125042507TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1762500.067.9733744
GTATGCC1765800.067.8853745
TATGCCG1763400.067.7673846
ATGCCGT1764000.067.6649647
AGTCACC1788200.067.6303328
ACGTCTG1802100.067.5820315
TCGTATG1773600.067.5696743
CCTTGTA1779200.067.56409533
CTCGTAT1748850.067.560742
GTCACCT1788600.067.52013429
ACACGTC1809450.067.5001213
GAACTCC1798300.067.4385421
CACGTCT1807950.067.4136914
CGTCTGA1807750.067.3931116
ACTCCAG1795500.067.37546523
CACCTTG1789850.067.367631
ACCTTGT1787050.067.3315332
GCACACG1817700.067.2994511
TGCCGTC1771850.067.2725548
CACACGT1819100.067.24209612