FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_AKM124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_AKM124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16735993
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC2311041.3808801186759578TruSeq Adapter, Index 11 (100% over 50bp)
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA280420.1675550414008897No Hit
CCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGC214710.12829235767486277No Hit
GCCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTG206400.12332701142979685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG254800.067.9370846
ATGCCGT258750.066.8599947
GCTACAT268300.063.85342835
CTACATC268550.063.55996736
CGGCTAC273000.063.203233
GGCTACA271700.063.1576134
GTATGCC274450.063.12375345
ACGGCTA280300.061.8689532
TGCCGTC281100.061.51948548
TACATCT282700.060.56448437
CGTATGC288100.060.18140444
GCCGTCT291850.059.25330449
CACGGCT297950.058.3331631
CTCGTAT302650.057.00008842
TCACGGC308000.056.46317330
TCTCGTA316650.054.39152541
TCGTATG321100.054.08365643
AAAAGGG301700.053.97828770
ACATCTC333750.051.46824338
CATCTCG349650.049.2180139
Produced by FastQC (version 0.11.5)