FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_AKM123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_AKM123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18217690
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC2747721.5082702581940959TruSeq Adapter, Index 10 (100% over 50bp)
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA284680.1562656955958741No Hit
CCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGC188130.10326775787709637No Hit
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTT186520.10238400148427161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG292100.067.4217746
ATGCCGT295350.065.95701647
TAGCTTA321650.065.4383334
ACTAGCT332050.063.599432
TATCTCG324200.063.19705639
GTATGCC318700.062.92542345
TGCCGTC311100.061.66190348
CTAGCTT343200.061.41095733
CACTAGC346850.061.05750331
CTTATCT339150.060.8550337
GTCACTA355850.059.84766429
CGTATGC335600.059.79820344
TTATCTC345250.059.749438
GCCGTCT323000.059.07559249
AGCTTAT357450.058.7961535
TCACTAG362850.058.59663830
CTCGTAT349300.057.77362442
TCGTATG361450.055.65714643
AGTCACT385400.055.4668828
AAAAGGG346450.054.8783870