Basic Statistics
Measure | Value |
---|---|
Filename | HH2GLBGXG_n01_AKM123.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 18217690 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC | 274772 | 1.5082702581940959 | TruSeq Adapter, Index 10 (100% over 50bp) |
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA | 28468 | 0.1562656955958741 | No Hit |
CCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGC | 18813 | 0.10326775787709637 | No Hit |
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTT | 18652 | 0.10238400148427161 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 29210 | 0.0 | 67.42177 | 46 |
ATGCCGT | 29535 | 0.0 | 65.957016 | 47 |
TAGCTTA | 32165 | 0.0 | 65.43833 | 34 |
ACTAGCT | 33205 | 0.0 | 63.5994 | 32 |
TATCTCG | 32420 | 0.0 | 63.197056 | 39 |
GTATGCC | 31870 | 0.0 | 62.925423 | 45 |
TGCCGTC | 31110 | 0.0 | 61.661903 | 48 |
CTAGCTT | 34320 | 0.0 | 61.410957 | 33 |
CACTAGC | 34685 | 0.0 | 61.057503 | 31 |
CTTATCT | 33915 | 0.0 | 60.85503 | 37 |
GTCACTA | 35585 | 0.0 | 59.847664 | 29 |
CGTATGC | 33560 | 0.0 | 59.798203 | 44 |
TTATCTC | 34525 | 0.0 | 59.7494 | 38 |
GCCGTCT | 32300 | 0.0 | 59.075592 | 49 |
AGCTTAT | 35745 | 0.0 | 58.79615 | 35 |
TCACTAG | 36285 | 0.0 | 58.596638 | 30 |
CTCGTAT | 34930 | 0.0 | 57.773624 | 42 |
TCGTATG | 36145 | 0.0 | 55.657146 | 43 |
AGTCACT | 38540 | 0.0 | 55.46688 | 28 |
AAAAGGG | 34645 | 0.0 | 54.87838 | 70 |