FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_AKM122.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_AKM122.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18076074
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC9041685.002015371258162TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG961800.068.62128446
ATGCCGT966100.068.29449547
GTATGCC983200.067.15298545
CGTATGC987450.066.98427644
TGCCGTC988550.066.7901148
GCCGTCT1004250.065.7178849
CTCGTAT993200.065.61388442
TCGTATG1012800.065.407943
CAGATCT1006600.064.2639137
TCTCGTA1013450.064.12343641
AAAAGGG975000.063.70633370
GATCAGA1035550.063.6564634
CAGTCAC1048600.063.49453727
TCAGATC1020350.063.40458336
ACGTCTG1054200.063.37986415
CGTCTGA1054250.063.35746416
CACACGT1056900.063.33960312
ATCAGAT1039650.063.23374635
GTCACGA1053050.063.0374729
TCACGAT1056650.062.89208630
Produced by FastQC (version 0.11.5)