FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_AKM117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_AKM117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15680370
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1728281.1021933793654104TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG201100.066.0644746
ATGCCGT206050.064.4775947
GTATGCC225250.059.1366145
TGCCGTC228100.058.2910648
CTGACCA231050.057.0909233
CGTATGC237650.056.18354444
GCCGTCT239050.055.6202549
ACTGACC239150.055.4062232
GACCAAT244200.053.47183635
TCACTGA253050.052.95764530
CTCGTAT248650.052.68477642
GTCACTG258100.052.38268729
AGTCACT259500.052.28839528
AAAAGGG245900.050.73840770
TGACCAA260600.050.2949434
ACCAATC257050.049.88685236
TCGTATG269950.049.68164443
TCTCGTA267850.048.725341
CACTGAC281600.047.4022631
CTTGAAA290500.046.14468460
Produced by FastQC (version 0.11.5)