FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_AKM116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_AKM116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15036856
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2303531.531922630635021TruSeq Adapter, Index 3 (100% over 50bp)
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG238930.15889624799226648No Hit
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA209670.1394373930295003No Hit
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA193640.12877691985611886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG251150.067.5869346
ATGCCGT254300.066.694947
ACTTAGG266950.064.83108532
TCACTTA268900.064.5305830
CACTTAG273150.063.50111431
CTTAGGC274100.063.00032833
GTATGCC274950.061.92745645
TGCCGTC275050.061.52341548
CGTATGC281100.060.58524344
GCCGTCT285000.059.3014249
TTAGGCA291650.058.96948234
CTCGTAT291800.057.81268342
GTCACTT309050.056.29472429
AGTCACT310050.056.2249228
AGGCATC305600.055.79701236
AAAAGGG285700.055.70228270
TCGTATG308750.055.10287543
TCTCGTA315000.053.53251341
TAGGCAT323200.053.12639635
CTTGAAA329450.051.2054360
Produced by FastQC (version 0.11.5)