FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_AKM115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_AKM115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19633316
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC4402192.2422040168863986TruSeq Adapter, Index 2 (100% over 50bp)
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG317010.16146533779622352No Hit
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA241440.12297464167540521No Hit
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA226860.11554848910902264No Hit
GGCGCTTGCTGAATTGGAATAGATCTTCCTTGAATCGCTTCCTTCTTTGG226030.11512573831134792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG464850.068.146446
ATGCCGT468550.067.3171247
TATCTCG491200.065.2453639
GTATGCC498500.064.2342445
TGCCGTC491950.063.73127748
CGTATGC509800.062.8583544
GTCACCG525400.062.28318429
TCACCGA526200.062.1083630
GCCGTCT505350.061.847649
CTCGTAT520050.061.49078442
AGTCACC537250.061.02667628
TGTATCT532900.060.4288237
GTATCTC537300.059.91426538
AAAAGGG506650.059.6272770
CACCGAT550250.059.49599531
ACCGATG549800.059.30263532
ATGTATC549250.059.06313736
TCGTATG543650.059.0410843
TCTCGTA552150.057.8908741
GATGTAT574350.057.41996835