FastQCFastQC Report
Tue 29 Sep 2020
HH2GLBGXG_n01_AKM114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GLBGXG_n01_AKM114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18465962
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC4340862.3507359107529844TruSeq Adapter, Index 1 (100% over 50bp)
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG267630.14493152319927877No Hit
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA204970.11099882042430284No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA195760.10601126548402948No Hit
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA193510.10479280743673142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG479250.067.8801746
ATGCCGT483400.067.2250147
GTATGCC506650.064.36874445
TGCCGTC510650.063.65839448
CGTATGC518100.063.04042444
GCCGTCT523550.062.08988649
ACATCAC529200.061.7429332
GTCACAT534150.061.53112429
CTCGTAT523400.061.37212442
AAAAGGG506300.060.16946870
TCGTATG547100.059.7564343
CATCACG551800.059.12549633
CACATCA558100.058.5522931
TCTCGTA546550.058.5228741
ATCACGA557100.058.07927334
TCACATC569500.057.50927730
CTTGAAA570600.057.18559360
TCACGAT563250.057.0662435
CGTCTGA583300.056.81439216
AGTCACA581500.056.700928